Generalised coherent point drift for group-wise registration of multi-dimensional point sets

In this paper we propose a probabilistic approach to group-wise registration of unstructured high-dimensional point sets. We focus on registration of generalised point sets which encapsulate both the positions of points on surface boundaries and corresponding normal vectors describing local surface geometry. Richer descriptions of shape can be especially valuable in applications involving complex and intricate variations in geometry, where spatial position alone is an unreliable descriptor for shape registration. A hybrid mixture model combining Student’s t and Von-Mises-Fisher distributions is proposed to model position and orientation components of the point sets, respectively. A group-wise rigid and non-rigid registration framework is then formulated on this basis. Two clinical data sets, comprising 27 brain ventricle and 15 heart shapes, were used to assess registration accuracy. Significant improvement in accuracy and anatomical validity of the estimated correspondences was achieved using the proposed approach, relative to state-of-the-art point set registration approaches, which consider spatial positions alone

Three-Dimensional Imaging of the Mouse Neurovasculature with Magnetic Resonance Microscopy

Knowledge of the three-dimensional (3D) architecture of blood vessels in the brain is crucial because the progression of various neuropathologies ranging from Alzheimer's disease to brain tumors involves anomalous blood vessels. The challenges in obtaining such data from patients, in conjunction with development of mouse models of neuropathology, have made the murine brain indispensable for investigating disease induced neurovascular changes. Here we describe a novel method for “whole brain” 3D mapping of murine neurovasculature using magnetic resonance microscopy (μMRI). This approach preserves the vascular and white matter tract architecture, and can be combined with complementary MRI contrast mechanisms such as diffusion tensor imaging (DTI) to examine the interplay between the vasculature and white matter reorganization that often characterizes neuropathologies. Following validation with micro computed tomography (μCT) and optical microscopy, we demonstrate the utility of this method by: (i) combined 3D imaging of angiogenesis and white matter reorganization in both, invasive and non-invasive brain tumor models; (ii) characterizing the morphological heterogeneity of the vascular phenotype in the murine brain; and (iii) conducting “multi-scale” imaging of brain tumor angiogenesis, wherein we directly compared in vivo MRI blood volume measurements with ex vivo vasculature data

PLoS One

Quantitative analysis of the vascular network anatomy is critical for the understanding of the vasculature structure and function. In this study, we have combined microcomputed tomography (microCT) and computational analysis to provide quantitative three-dimensional geometrical and topological characterization of the normal kidney vasculature, and to investigate how 2 core genes of the Wnt/planar cell polarity, Frizzled4 and Frizzled6, affect vascular network morphogenesis. Experiments were performed on frizzled4 (Fzd4-/-) and frizzled6 (Fzd6-/-) deleted mice and littermate controls (WT) perfused with a contrast medium after euthanasia and exsanguination. The kidneys were scanned with a high-resolution (16 μm) microCT imaging system, followed by 3D reconstruction of the arterial vasculature. Computational treatment includes decomposition of 3D networks based on Diameter-Defined Strahler Order (DDSO). We have calculated quantitative (i) Global scale parameters, such as the volume of the vasculature and its fractal dimension (ii) Structural parameters depending on the DDSO hierarchical levels such as hierarchical ordering, diameter, length and branching angles of the vessel segments, and (iii) Functional parameters such as estimated resistance to blood flow alongside the vascular tree and average density of terminal arterioles. In normal kidneys, fractal dimension was 2.07±0.11 (n = 7), and was significantly lower in Fzd4-/- (1.71±0.04; n = 4), and Fzd6-/- (1.54±0.09; n = 3) kidneys. The DDSO number was 5 in WT and Fzd4-/-, and only 4 in Fzd6-/-. Scaling characteristics such as diameter and length of vessel segments were altered in mutants, whereas bifurcation angles were not different from WT. Fzd4 and Fzd6 deletion increased vessel resistance, calculated using the Hagen-Poiseuille equation, for each DDSO, and decreased the density and the homogeneity of the distal vessel segments. Our results show that our methodology is suitable for 3D quantitative characterization of vascular networks, and that Fzd4 and Fzd6 genes have a deep patterning effect on arterial vessel morphogenesis that may determine its functional efficiency

Thermotectonic evolution of an extensional dome : the cenozoic Osogovo-Lisets core complex (Kraishte zone, western Bulgaria)

The Kraishte region of Bulgaria is located at the junction of the Balkanides and Hellenides-Dinarides tectonic belts. Fission-track analysis on both apatites and zircons documents the Cenozoic exhumation of a Precambrian basement bounded by low-angle detachments. Late Eocene - Oligocene extension began prior to 47 Ma and was dominantly in a top-to-the-southwest direction, confirmed by the sense of younging of apatite and zircon ages. This crustal extension controlled the formation of half-graben sedimentary basins on the hanging walls of the detachments. Thermal modelling of these hanging wall units provides evidence for heat transfer across the detachments from a relatively warm rising footwall. From 32 to 29 Ma, pervasive magmatic activity resulted in the emplacement of rhyolitic to dacitic subvolcanic bodies and dykes, along with intrusion of the Osogovo granite. The results give evidence for extension in the southern Balkan older than, and separated from, the Miocene to Quaternary Aegean extension. This might reflect transtension during northeastward extrusion and rotation of continental fragments around the western boundary of Moesia. Eocene - Oligocene extension seems to have been controlled by the distribution of earlier thickening all around the Carpatho-Balkanic orocline, which is reflected by the Cretaceous emplacement of the Morava Nappe in the Kraishte

Agricultural Academy

Abstract NEOV, B., D. TEOFANOVA, L. ZAGORCHEV, G. RADOSLAVOV and P. HRISTOV, 2013. Mi lk protein polymorphism in Bulgarian grey cattle population. Bulg. J. Agric. Sci., Supplement 2, Application of gene specifi c molecular markers in genotyping and genetic identifi cation is of an essential signifi cance for preserving genetic diversity. The aim of the present research is to reveal the genotype profi le of native Bulgarian Grey cattle population with respect to lactoprotein genes: kappa-casein and αS1-casein through PCR-RFLP analysis. The results for kappa-casein locus show superiority of heterozygous AB genotype and prevalence of the uncommon B allele. Analysis of the polymorphism of alfaS1-casein gene displays high frequency of heterozygous BC genotype and C allele of gene. Genetic profi le of the breed is compared to other cattle populations to establish the position of the breed about other European cattle breeds genetic diversity. It may be concluded that Bulgarian grey cow is with specifi c genotype profi le similar to other cattle population in South-Eastern Europe

Middle Triassic palaeomagnetic data from northern Bulgaria: constraints on Tethyan magnetostratigraphy and palaeogeography

Magnetostratigraphic and biostratigraphic data are presented from the Anisian (Middle Triassic) Peri-Tethyan Edivetur section of northwestern Bulgaria. A dual-polarity component of magnetization carried by magnetite delineates a magnetic stratigraphy of mainly reversed polarity. Magnetozones are dated by means of foraminifer and conodont biostratigraphy. Data from Edivetur are compared with data from Middle Triassic Tethyan limestone sections with the aim of contributing to the completion of the Middle Triassic magnetic polarity time scale. We also propose that paleomagnetic data from Edivetur can be used as proxy data for the paleogeographic position of the Moesian platform. The Moesian platform was located at 21-24\ub0N along the southern margin of Europe. It was probably marginally separated, but not detached or rotated away from Europe by the North Dobrugea transtensional trough, which is interpreted as a back-arc basin resulting from the northward subduction of the Neo-Tethys (Vardar) or Paleo-Tethys ocean. Paleomagnetic data from this study and other minor tectonic elements are used to generate a paleogeographic sketch map of the Pangea-bounded western Tethys and Peri-Tethys at Middle/early Late Triassic time. (C) 2000 Elsevier Science B.V